Abstract
FF6 tumor cells are derived from a spontaneous rat squamous cell carcinoma (SCC) which originally arose in the facial skin of a DA rat. In this study, FF6 tumor cells were implanted into rat oral mucosa to establish an ex vivo metastatic model. We analyzed the expression of intercellular cell adhesion molecule-1 (ICAM-1) in the implanted primary and metastatic FF6 tumors by immuno-staining with a monoclonal antibody (mAb) against ICAM-1. The implanted primary FF6 cells showed strong expression of ICAM-1, whereas the tumor cells of metastatic lesions showed weak or negative expression of ICAM-1. By immunostaining with mAb OX6, a number of MHC class II-positive macrophages were detected in tumor mesenchyme and surrounding the metastatic foci. These results suggested that the local immune reaction in the lymph node influenced the expression of ICAM-1 on tumor cells, and that MHC class II-positive macrophages may play a role in transplanted tumor growth and metastases.
MeSH terms
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Animals
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Antibodies, Monoclonal
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CD4 Antigens / analysis
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CD4-Positive T-Lymphocytes / pathology
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CD8 Antigens / analysis
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CD8-Positive T-Lymphocytes / pathology
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Carcinoma, Squamous Cell / pathology*
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Carcinoma, Squamous Cell / secondary
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Coloring Agents
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Eosine Yellowish-(YS)
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Facial Neoplasms / pathology
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Fluorescent Dyes
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Gene Expression Regulation, Neoplastic
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Hematoxylin
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Histocompatibility Antigens Class II / analysis
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Immunoenzyme Techniques
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Intercellular Adhesion Molecule-1 / analysis*
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Intercellular Adhesion Molecule-1 / genetics
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Lip Neoplasms / pathology
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Lymph Nodes / immunology
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Lymph Nodes / pathology
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Lymphatic Metastasis / pathology
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Macrophages / immunology
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Macrophages / pathology
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Mesoderm / pathology
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Mouth Neoplasms / pathology
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Mouth Neoplasms / secondary
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Neoplasm Transplantation
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Random Allocation
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Rats
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Rats, Inbred Strains
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Tongue Neoplasms / pathology
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Tumor Cells, Cultured
Substances
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Antibodies, Monoclonal
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CD4 Antigens
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CD8 Antigens
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Coloring Agents
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Fluorescent Dyes
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Histocompatibility Antigens Class II
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Intercellular Adhesion Molecule-1
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Eosine Yellowish-(YS)
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Hematoxylin