Two novel lipases from thermophile Bacillus thermocatenulatus: screening, purification, cloning, overexpression, and properties

Methods Enzymol. 1997:284:194-220. doi: 10.1016/s0076-6879(97)84013-x.
No abstract available

Publication types

  • Comparative Study

MeSH terms

  • Amino Acid Sequence
  • Amino Acids / analysis
  • Bacillus / enzymology*
  • Base Sequence
  • Chromatography, Ion Exchange
  • Cloning, Molecular / methods
  • Culture Media
  • Electrophoresis, Polyacrylamide Gel
  • Escherichia coli
  • Indicators and Reagents
  • Kinetics
  • Lipase / chemistry*
  • Lipase / isolation & purification
  • Lipase / metabolism*
  • Molecular Sequence Data
  • Molecular Weight
  • Plasmids
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / isolation & purification
  • Recombinant Proteins / metabolism
  • Restriction Mapping
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Species Specificity

Substances

  • Amino Acids
  • Culture Media
  • Indicators and Reagents
  • Recombinant Proteins
  • Lipase