A TLC method was used in the identification of the following chemical consitituents of medicinal herbs in Tanre Kesou Granules: Radix Scutellariae, Bulbus Fritillariae Thunbergii, Pericarpium Citri Reticulatae and Fructus Aurantii Immaturus. The sample was purified through acidification with hydrochloric acid and extraction with ethyl acetatethe. The contents of baicalin in the preparation and Radix Scutellariae were determined by means of HPLC and TLC-densitometry. These methods fit for quality control of the preparation containing baicalin.