Identification of clonogenic common lymphoid progenitors in mouse bone marrow

Cell. 1997 Nov 28;91(5):661-72. doi: 10.1016/s0092-8674(00)80453-5.

Abstract

The existence of a common lymphoid progenitor that can only give rise to T cells, B cells, and natural killer (NK) cells remains controversial and constitutes an important gap in the hematopoietic lineage maps. Here, we report that the Lin(-)IL-7R(+)Thy-1(-)Sca-1loc-Kit(lo) population from adult mouse bone marrow possessed a rapid lymphoid-restricted (T, B, and NK) reconstitution capacity in vivo but completely lacked myeloid differentiation potential either in vivo or in vitro. A single Lin(-)IL-7R(+)Thy-1(-)Sca-1loc-Kit(lo) cell could generate at least both T and B cells. These data provide direct evidence for the existence of common lymphoid progenitors in sites of early hematopoiesis.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antigens, Ly / analysis
  • B-Lymphocytes / cytology
  • Cell Differentiation / physiology
  • Clone Cells / chemistry
  • Clone Cells / cytology
  • Flow Cytometry
  • Hematopoietic Stem Cells / chemistry*
  • Hematopoietic Stem Cells / cytology*
  • Indicator Dilution Techniques
  • Interleukin-7 / analysis
  • Membrane Proteins / analysis
  • Methylcellulose
  • Mice
  • Mice, Inbred C57BL
  • Proto-Oncogene Proteins c-kit / analysis
  • T-Lymphocytes / cytology
  • Thy-1 Antigens / analysis

Substances

  • Antigens, Ly
  • Interleukin-7
  • Ly6a protein, mouse
  • Membrane Proteins
  • Thy-1 Antigens
  • Methylcellulose
  • Proto-Oncogene Proteins c-kit