Abstract
A Sonic hedgehog (Shh) response element was identified in the chicken ovalbumin upstream promoter-transcription factor II (COUP-TFII) promoter that binds to a factor distinct from Gli, a gene known to mediate Shh signaling. Although this binding activity is specifically stimulated by Shh-N (amino-terminal signaling domain), it can also be unmasked with protein phosphatase treatment in the mouse cell line P19, and induction by Shh-N can be blocked by phosphatase inhibitors. Thus, Shh-N signaling may result in dephosphorylation of a target factor that is required for activation of COUP-TFII-, Islet1-, and Gli response element-dependent gene expression. This finding identifies another step in the Shh-N signaling pathway.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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COUP Transcription Factor II
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COUP Transcription Factors
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Cell Line
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DNA / metabolism
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DNA-Binding Proteins / genetics*
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DNA-Binding Proteins / metabolism
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Enzyme Inhibitors / pharmacology
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Gene Expression Regulation*
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Hedgehog Proteins
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Marine Toxins
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Mice
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Okadaic Acid / pharmacology
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Oxazoles / pharmacology
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Phosphoprotein Phosphatases / antagonists & inhibitors
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Phosphoprotein Phosphatases / metabolism*
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Phosphorylation
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Promoter Regions, Genetic
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Proteins / genetics*
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Proteins / metabolism*
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Receptors, Steroid*
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Signal Transduction
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Trans-Activators*
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Transcription Factors / genetics*
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Transcription Factors / metabolism
Substances
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COUP Transcription Factor II
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COUP Transcription Factors
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DNA-Binding Proteins
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Enzyme Inhibitors
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Hedgehog Proteins
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Marine Toxins
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Nr2f2 protein, mouse
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Oxazoles
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Proteins
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Receptors, Steroid
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Trans-Activators
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Transcription Factors
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Okadaic Acid
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calyculin A
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DNA
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Phosphoprotein Phosphatases