Abstract
P-selectin is one of the key early mediators of leukocyte adhesion in inflammatory conditions. This report examines the role of P-selectin in a neutrophil- and platelet-mediated model of glomerulonephritis (the concanavalin A [con A] model). The administration of neutralizing anti-P-selectin antibody (PB 1.3) reduced the platelet influx at 10 min (P < 0.05) and was associated with a 60% reduction in the neutrophil infiltrate and a 50% reduction in the number of oxidant-producing cells at 3 h within glomeruli. No effect on glomerular monocyte-macrophage accumulation was observed, and proteinuria was reduced by 20% but did not reach significance. It is concluded that P-selectin plays an important role in mediating the neutrophil and platelet accumulation in this model and likely has a role in mediating the glomerular injury.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Antibodies, Monoclonal / immunology
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Antibodies, Monoclonal / pharmacology
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Antibodies, Monoclonal / therapeutic use
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Blood Platelets / pathology*
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Cell Adhesion
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Chemotaxis, Leukocyte
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Complement C3 / analysis
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Concanavalin A / administration & dosage
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Concanavalin A / immunology
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Concanavalin A / toxicity
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Endothelium, Vascular / injuries
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Endothelium, Vascular / pathology
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Glomerulonephritis / chemically induced
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Glomerulonephritis / pathology*
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Glomerulonephritis / prevention & control
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Immune Complex Diseases / chemically induced
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Immune Complex Diseases / pathology*
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Immune Complex Diseases / prevention & control
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Immunoglobulin G / immunology
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Immunoglobulin G / toxicity
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Infusions, Intra-Arterial
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Kidney Glomerulus / pathology
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Male
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Neutrophils / pathology*
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P-Selectin / immunology
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P-Selectin / physiology*
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Platelet Adhesiveness
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Rats
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Rats, Sprague-Dawley
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Reactive Oxygen Species
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Renal Artery
Substances
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Antibodies, Monoclonal
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Complement C3
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Immunoglobulin G
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P-Selectin
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Reactive Oxygen Species
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Concanavalin A