A role for P-selectin in neutrophil and platelet infiltration in immune complex glomerulonephritis

J Am Soc Nephrol. 1997 Dec;8(12):1838-44. doi: 10.1681/ASN.V8121838.

Abstract

P-selectin is one of the key early mediators of leukocyte adhesion in inflammatory conditions. This report examines the role of P-selectin in a neutrophil- and platelet-mediated model of glomerulonephritis (the concanavalin A [con A] model). The administration of neutralizing anti-P-selectin antibody (PB 1.3) reduced the platelet influx at 10 min (P < 0.05) and was associated with a 60% reduction in the neutrophil infiltrate and a 50% reduction in the number of oxidant-producing cells at 3 h within glomeruli. No effect on glomerular monocyte-macrophage accumulation was observed, and proteinuria was reduced by 20% but did not reach significance. It is concluded that P-selectin plays an important role in mediating the neutrophil and platelet accumulation in this model and likely has a role in mediating the glomerular injury.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Monoclonal / immunology
  • Antibodies, Monoclonal / pharmacology
  • Antibodies, Monoclonal / therapeutic use
  • Blood Platelets / pathology*
  • Cell Adhesion
  • Chemotaxis, Leukocyte
  • Complement C3 / analysis
  • Concanavalin A / administration & dosage
  • Concanavalin A / immunology
  • Concanavalin A / toxicity
  • Endothelium, Vascular / injuries
  • Endothelium, Vascular / pathology
  • Glomerulonephritis / chemically induced
  • Glomerulonephritis / pathology*
  • Glomerulonephritis / prevention & control
  • Immune Complex Diseases / chemically induced
  • Immune Complex Diseases / pathology*
  • Immune Complex Diseases / prevention & control
  • Immunoglobulin G / immunology
  • Immunoglobulin G / toxicity
  • Infusions, Intra-Arterial
  • Kidney Glomerulus / pathology
  • Male
  • Neutrophils / pathology*
  • P-Selectin / immunology
  • P-Selectin / physiology*
  • Platelet Adhesiveness
  • Rats
  • Rats, Sprague-Dawley
  • Reactive Oxygen Species
  • Renal Artery

Substances

  • Antibodies, Monoclonal
  • Complement C3
  • Immunoglobulin G
  • P-Selectin
  • Reactive Oxygen Species
  • Concanavalin A