Characterization and sensitivity to interleukin 2 and interferon alpha of leukemic cells from a patient with large granular lymphocytic leukemia associated with chronic active Epstein-Barr virus infection

Leuk Res. 1997 Oct;21(10):941-50. doi: 10.1016/s0145-2126(97)00057-x.

Abstract

A patient presented with chronic large granular lymphocyte leukemia associated with chronic active Epstein-Barr virus infection (CAEBV). Cell cycle analysis revealed a minimal growth compatible with chronic lymphocytic leukemia After 5 months of treatment, the patient died from acute transformation of the leukemia. Cell harvested during chronic phase were analyzed for sensitivity to interleukin 2 (IL-2) and interferon alpha (IFN alpha) in vitro by means of surface phenotyping and cell cycle assay. IL-2 induced remarkable growth of the cells, whereas IFN alpha did not confer a growth advantage. Since IFN alpha was expected to have no growth induction effect on the leukemia cells, it was administered to the patient to treat the CAEBV.

MeSH terms

  • Antineoplastic Agents / pharmacology*
  • Antiviral Agents / pharmacology*
  • Cell Cycle
  • DNA, Viral / chemistry
  • Female
  • Herpesviridae Infections / complications*
  • Herpesvirus 4, Human* / genetics
  • Humans
  • Interferon-alpha / pharmacology*
  • Interleukin-2 / pharmacology*
  • Leukemia, Lymphoid / complications
  • Leukemia, Lymphoid / virology*
  • Phenotype
  • Tumor Cells, Cultured
  • Tumor Virus Infections / complications*

Substances

  • Antineoplastic Agents
  • Antiviral Agents
  • DNA, Viral
  • Interferon-alpha
  • Interleukin-2