Abstract
Stress controls the expression of a cohort of genes. Among these, the glucose-regulated protein (GRP) genes are specifically activated by glucose deprivation, reducing agents, glycosylation block, intracellular calcium or ex vivo incubations of tissues or cells. We demonstrate that these stimuli induce the expression of the cytosolic aspartate aminotransferase gene in adipocytes by a process involving the region of the promoter between -2405 and -26 bp. Therefore this transaminase is a new member of the GRP family.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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3T3 Cells
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Adipocytes / chemistry
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Adipocytes / enzymology*
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Animals
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Aspartate Aminotransferases / genetics*
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Blotting, Northern
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Calcimycin / pharmacology
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Chloramphenicol O-Acetyltransferase / analysis
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Cytosol / enzymology
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Gene Expression Regulation, Enzymologic
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Genes, Reporter
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Glucose / metabolism
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HSP70 Heat-Shock Proteins / genetics*
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Hot Temperature
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Membrane Proteins / genetics*
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Mercaptoethanol / pharmacology
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Mice
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Promoter Regions, Genetic
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Stress, Physiological
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Transfection
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Tunicamycin / pharmacology
Substances
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HSP70 Heat-Shock Proteins
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Membrane Proteins
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RNA, Messenger
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glucose-regulated proteins
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Tunicamycin
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Calcimycin
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Mercaptoethanol
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Chloramphenicol O-Acetyltransferase
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Aspartate Aminotransferases
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Glucose