Fast isolation of RNA to detect expression of tumor markers

J Clin Lab Anal. 1997;11(6):340-2. doi: 10.1002/(SICI)1098-2825(1997)11:6<340::AID-JCLA5>3.0.CO;2-9.

Abstract

The expression status of several tumor-related proteins is of great interest in clinical examination and research. As a completion to conventional antibody staining, RT-PCR is often used today. Reliable isolation of RNA from a low number of cells is very often a critical stage of such an examination. We demonstrate here a simple and fast method to isolate RNA from only 10,000 cells and applied it to the detection of CEA, c-ERB-B2, and mdr-1 as often studied models for tumor markers.

MeSH terms

  • ATP Binding Cassette Transporter, Subfamily B, Member 1 / analysis
  • ATP Binding Cassette Transporter, Subfamily B, Member 1 / genetics
  • Biomarkers, Tumor / analysis*
  • Carcinoembryonic Antigen / analysis
  • Carcinoembryonic Antigen / genetics
  • Gene Expression
  • Humans
  • RNA, Neoplasm / isolation & purification*
  • RNA-Directed DNA Polymerase
  • Receptor, ErbB-2 / analysis
  • Receptor, ErbB-2 / genetics
  • Tumor Cells, Cultured

Substances

  • ATP Binding Cassette Transporter, Subfamily B, Member 1
  • Biomarkers, Tumor
  • Carcinoembryonic Antigen
  • RNA, Neoplasm
  • Receptor, ErbB-2
  • RNA-Directed DNA Polymerase