Different expression of adhesion molecules on myeloid and B-lymphoid CD34+ progenitors in normal bone marrow

Eur J Haematol. 1997 Nov;59(5):277-86. doi: 10.1111/j.1600-0609.1997.tb01687.x.

Abstract

The expression of adhesion molecules was studied on B lymphoid and myeloid CD34+ precursors in normal bone marrow. Bone marrow aspirates were labelled in a double fluorescence procedure with the CD34 monoclonal antibody 43A1 and with antibodies directed against maturation and differentiation antigens and adhesion molecules. Three clusters of CD34+ cells could be distinguished by their light scatter characteristics in flow cytometry. The population with the lowest forward scatter contained B-lymphoid precursors while the two others showed phenotypic characteristics of, respectively, early and late myeloid precursors. Nearly all CD34+ cells in the 3 subpopulations expressed VLA-4, VLA-5, LFA-3 and H-CAM. B-lymphoid progenitors showed a higher density of VLA-4 and VLA-5 than the myeloid progenitors. Myeloid precursors, and particularly the late subset, expressed more HCAM than the B-lymphoid progenitors. The majority of the CD34+ cells also expressed LFA-1 and L-selectin. Higher numbers of positive cells were found in the myeloid subset. The early myeloid subset showed the highest positivity for L-selectin. We conclude that B lymphoid and early and late myeloid CD34+ precursors in normal bone marrow show a different profile of adhesion molecules. These profiles could reflect a higher tendency of the myeloid CD34+ precursors to circulate.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Antigens, CD / analysis
  • Antigens, CD34 / analysis*
  • B-Lymphocytes / chemistry*
  • B-Lymphocytes / immunology
  • Bone Marrow Cells / chemistry*
  • Bone Marrow Cells / immunology
  • CD58 Antigens / analysis
  • Cell Adhesion Molecules / analysis*
  • Granulocytes / chemistry*
  • Granulocytes / immunology
  • Hematopoietic Stem Cells / chemistry*
  • Hematopoietic Stem Cells / immunology
  • Humans
  • Hyaluronan Receptors / analysis
  • Integrin alpha4beta1
  • Integrins / analysis
  • L-Selectin / analysis
  • Receptors, Fibronectin / analysis
  • Receptors, Lymphocyte Homing / analysis

Substances

  • Antigens, CD
  • Antigens, CD34
  • CD58 Antigens
  • Cell Adhesion Molecules
  • Hyaluronan Receptors
  • Integrin alpha4beta1
  • Integrins
  • Receptors, Fibronectin
  • Receptors, Lymphocyte Homing
  • L-Selectin