High-level overexpression of yeast elongation factor 3 and detailed kinetic analysis using a coupled spectrophotometric assay

Anal Biochem. 1997 Dec 15;254(2):288-90. doi: 10.1006/abio.1997.2456.
No abstract available

MeSH terms

  • Adenosine Triphosphate / metabolism
  • Alcohol Dehydrogenase / genetics
  • Blotting, Western
  • Cloning, Molecular
  • Fungal Proteins*
  • Kinetics
  • Peptide Elongation Factors / genetics
  • Peptide Elongation Factors / metabolism*
  • Promoter Regions, Genetic / genetics
  • Saccharomyces cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Spectrophotometry / methods*

Substances

  • Fungal Proteins
  • Peptide Elongation Factors
  • Saccharomyces cerevisiae Proteins
  • YEF3 protein, S cerevisiae
  • Adenosine Triphosphate
  • Alcohol Dehydrogenase