Abstract
To determine whether Helicobacter pylori infection affects clearance of a concomitant viral infection and cytotoxic T lymphocyte (CTL) and cytokine response to that infection, H. pylori-infected BALB/c mice were challenged with a recombinant vaccinia virus expressing human immunodeficiency virus type 1 gp160. Two H. pylori strains, a colonizing clinical isolate (KS612) and an established standard noncolonizing strain (NCTC11637), were compared. Clearance of recombinant vaccinia virus was reduced in KS612-infected mice compared with NCTC11637-infected and control mice. As a potential mechanism, in contrast to control or NCTC11637-infected mice, the H. pylori clinical isolate KS612 diminished gp160-specific and vaccinia virus-specific CTL activity, even in the presence of exogenous interleukin-2. Furthermore, KS612-infected mice had reduced Th1 cytokine responses to gp120 in vitro compared with control or NCTC11637-infected mice. These results have implications for possible effects of prevalent H. pylori infection on other human diseases.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antigens, Bacterial / immunology
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CD4-Positive T-Lymphocytes / immunology
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CD4-Positive T-Lymphocytes / metabolism
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CD8-Positive T-Lymphocytes / immunology
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CD8-Positive T-Lymphocytes / metabolism
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Cytotoxicity Tests, Immunologic
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Female
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HIV / physiology
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HIV Envelope Protein gp160 / genetics
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HIV Envelope Protein gp160 / immunology
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HIV Infections / complications*
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HIV Infections / genetics
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HIV Infections / immunology*
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Helicobacter Infections / complications*
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Helicobacter Infections / immunology*
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Helicobacter pylori / immunology
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Helicobacter pylori / pathogenicity
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Interferon-gamma / immunology
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Interferon-gamma / metabolism
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Interleukin-2 / immunology
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Interleukin-2 / metabolism
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Interleukin-2 / pharmacology
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Interleukin-4 / immunology
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Interleukin-4 / metabolism
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Liver / virology
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Mice
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Mice, Inbred BALB C
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Recombination, Genetic
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Specific Pathogen-Free Organisms
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Spleen / immunology
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Spleen / virology
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T-Lymphocytes, Cytotoxic / immunology*
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Vaccinia virus / genetics
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Virus Latency
Substances
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Antigens, Bacterial
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HIV Envelope Protein gp160
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Interleukin-2
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Interleukin-4
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Interferon-gamma