Abstract
Intracellular localization of organelles may depend in part on specific cytoplasmic linker proteins (CLIPs) that link membranous organelles to microtubules. Here, we characterize rat cDNAs encoding a novel, brain-specific CLIP of 115 kDa. This protein contains two N-terminal microtubule-binding domains and a long coiled-coil region; it binds to microtubules and is homologous to CLIP-170, a protein mediating the binding of endosomes to microtubules. CLIP-115 is enriched in the dendritic lamellar body (DLB), a recently discovered organelle predominantly present in bulbous dendritic appendages of neurons linked by dendrodendritic gap junctions. Local microtubule depolymerization leads to a temporary reduction of DLBs. These results suggest that CLIP-115 operates in the control of brain-specific organelle translocations.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Brain / cytology
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Brain / metabolism*
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COS Cells
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Cloning, Molecular
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Dendrites / metabolism*
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Dendrites / ultrastructure
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Humans
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Male
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Microtubule-Associated Proteins / analysis
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Microtubule-Associated Proteins / biosynthesis*
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Microtubule-Associated Proteins / chemistry
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Microtubules / drug effects
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Microtubules / metabolism
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Microtubules / ultrastructure
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Molecular Sequence Data
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Neoplasm Proteins
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Nerve Tissue Proteins / analysis
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Nerve Tissue Proteins / biosynthesis*
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Nerve Tissue Proteins / chemistry
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Neurons / cytology
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Neurons / drug effects
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Neurons / metabolism*
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Nocodazole / pharmacology
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Olivary Nucleus / metabolism
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Paclitaxel / pharmacology
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Rats
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Rats, Wistar
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Recombinant Proteins / analysis
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Recombinant Proteins / biosynthesis
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Sequence Alignment
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Sequence Homology, Amino Acid
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Transfection
Substances
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Microtubule-Associated Proteins
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Neoplasm Proteins
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Nerve Tissue Proteins
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Recombinant Proteins
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cytoplasmic linker protein 115
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cytoplasmic linker protein 170
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Paclitaxel
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Nocodazole