Abstract
Circulating lymphocytes are recruited from the blood to the tissue by rolling along the endothelium until being stopped by a signaling event linked to the Gialpha subunit of a heterotrimeric GTP-binding protein; that event then triggers rapid integrin-dependent adhesion. Four chemokines are now shown to induce such adhesion to intercellular adhesion molecule-1 and to induce arrest of rolling cells within 1 second under flow conditions similar to those of blood. SDF-1 (also called PBSF), 6-C-kine (also called Exodus-2), and MIP-3beta (also called ELC or Exodus-3) induced adhesion of most circulating lymphocytes, including most CD4+ T cells; and MIP-3alpha (also called LARC or Exodus-1) triggered adhesion of memory, but not naïve, CD4+ T cells. Thus, chemokines can regulate the arrest of lymphocyte subsets under flowing conditions, which may allow them to control lymphocyte-endothelial cell recognition and lymphocyte recruitment in vivo.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Antigens, Surface / metabolism
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CD4-Positive T-Lymphocytes / physiology*
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Cell Adhesion*
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Chemokine CCL19
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Chemokine CCL20
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Chemokine CCL21
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Chemokine CXCL12
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Chemokines, CC / pharmacology*
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Chemokines, CC / physiology
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Chemokines, CXC*
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Humans
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Immunologic Memory
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Intercellular Adhesion Molecule-1 / metabolism
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Lymphocytes / physiology*
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Macrophage Inflammatory Proteins*
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Membrane Proteins
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Receptors, CCR6
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Receptors, Chemokine*
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Rheology
Substances
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Antigens, Surface
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CCL19 protein, human
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CCL20 protein, human
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CCL21 protein, human
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CCR6 protein, human
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CXCL12 protein, human
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Chemokine CCL19
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Chemokine CCL20
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Chemokine CCL21
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Chemokine CXCL12
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Chemokines, CC
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Chemokines, CXC
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L-selectin counter-receptors
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Macrophage Inflammatory Proteins
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Membrane Proteins
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Receptors, CCR6
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Receptors, Chemokine
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Intercellular Adhesion Molecule-1