Purification of Pf155/RESA antigen from supernatants of in vitro Plasmodium falciparum cultures by continuous elution electrophoresis

B Dubois; P Deloron

doi:10.1016/s0022-1759(97)00122-1

Purification of Pf155/RESA antigen from supernatants of in vitro Plasmodium falciparum cultures by continuous elution electrophoresis

J Immunol Methods. 1997 Oct 13;208(1):29-34. doi: 10.1016/s0022-1759(97)00122-1.

Authors

B Dubois¹, P Deloron

Affiliation

¹ Institut National de la Santé et de la Recherche Médicale (INSERM) Unité 13, Paris, France.

PMID: 9433457
DOI: 10.1016/s0022-1759(97)00122-1

Abstract

The malaria parasite Plasmodium falciparum synthesizes a protein, Pf155/RESA, which associates with the membrane of newly invaded erythrocytes. Using spent supernatants from P. falciparum growing in culture as a source of soluble Pf155/RESA, we have developed a purification technique based on the concentration of these supernatants, followed by gel filtration and continuous elution electrophoresis. SDS-PAGE electrophoresis and immunoblots were used to establish the quality of the purification.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antigens, Protozoan / isolation & purification*
Antigens, Surface / isolation & purification*
Chromatography, Gel
Culture Media / chemistry
Electrophoresis, Polyacrylamide Gel
Immunoblotting
Plasmodium falciparum / chemistry
Plasmodium falciparum / immunology*
Protozoan Proteins / isolation & purification*

Substances

Antigens, Protozoan
Antigens, Surface
Culture Media
Protozoan Proteins
ring-infected erythrocyte surface antigen (RESA), Plasmodium falciparum