Abstract
We have developed a new and simple system of human osteoclast formation by fusing peripheral blood monocytes with anti-Fusion Regulatory Protein-1 (anti-FRP-1) monoclonal antibody (mAb). When human blood monocytes were cultured in the presence of anti-FRP-1/CD98 mAbs, polykaryocytes began to appear at approximately 15 h and increased in size with time until 3-4 days of incubation with anti-FRP-1 mAb. These fused cells showed positive staining in tartrate-resistant acid phosphatase, possessed numerous calcitonin receptors, and were capable of bone resorption. These results strongly suggest that anti-FRP-1 antibody-induced multinucleated cells are osteoclasts. Furthermore, FRP-1 antigens were detected in osteoclasts isolated from human bone and in the osteoclast-like cells obtained from human giant cell tumors of bone.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acid Phosphatase
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Amino Acids / analysis
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Antibodies, Monoclonal / pharmacology*
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Antigens, CD / analysis
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Antigens, CD / immunology*
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Antigens, Surface / analysis
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Carrier Proteins / analysis
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Carrier Proteins / immunology*
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Cells, Cultured
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Child
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Femur / cytology
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Fusion Regulatory Protein-1
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Giant Cell Tumors / immunology
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Giant Cell Tumors / pathology
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Giant Cells / immunology
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Giant Cells / pathology
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Humans
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Isoenzymes
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Monocytes / cytology*
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Monocytes / immunology*
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Osteoclasts / cytology*
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Osteoclasts / immunology*
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Receptors, Calcitonin / analysis
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Staining and Labeling
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Tartrate-Resistant Acid Phosphatase
Substances
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Amino Acids
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Antibodies, Monoclonal
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Antigens, CD
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Antigens, Surface
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Carrier Proteins
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Fusion Regulatory Protein-1
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Isoenzymes
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Receptors, Calcitonin
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pyridinoline
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Acid Phosphatase
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Tartrate-Resistant Acid Phosphatase