Expression and regulation of galanin-R2 receptors in rat primary sensory neurons: effect of axotomy and inflammation

Neurosci Lett. 1997 Nov 21;237(2-3):57-60. doi: 10.1016/s0304-3940(97)00805-7.

Abstract

Using in situ hybridization, we studied galanin-R2 receptor (GAL-R2-R) mRNA in rat lumbar 5 dorsal root ganglia (DRGs) at different time points after peripheral tissue inflammation and sciatic nerve transection (axotomy). About 25% of all normal DRG neuron profiles were GAL-R2-R mRNA-positive, and the majority was of the small type. In normal DRGs GAL-R2-R mRNA often (approximately 80%) colocalized with CGRP mRNA and sometimes (approximately 20%) with GAL-R1-R mRNA. There was a strong increase in the number and labeling intensity of GAL-R2-R mRNA-positive neuron profiles after peripheral tissue inflammation with a peak at 3 days, as well as a long-lasting decrease after axotomy. These results, together with the previously shown regulation of GAL and the GAL-R1-R, suggest that GALergic mechanisms participate in complex adaptive responses in DRGs after inflammation and nerve injury.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Axotomy
  • Calcitonin Gene-Related Peptide / biosynthesis
  • GTP-Binding Proteins / biosynthesis*
  • GTP-Binding Proteins / metabolism*
  • Ganglia, Spinal / cytology
  • Ganglia, Spinal / metabolism
  • In Situ Hybridization
  • Neuritis / metabolism*
  • Neurons, Afferent / metabolism*
  • Oligonucleotide Probes
  • RNA, Messenger / biosynthesis
  • Rats
  • Rats, Sprague-Dawley
  • Receptors, Galanin
  • Receptors, Gastrointestinal Hormone / biosynthesis*
  • Receptors, Gastrointestinal Hormone / metabolism*

Substances

  • Oligonucleotide Probes
  • RNA, Messenger
  • Receptors, Galanin
  • Receptors, Gastrointestinal Hormone
  • GTP-Binding Proteins
  • Calcitonin Gene-Related Peptide