A new transformation-deficient mutant of Haemophilus influenzae Rd with normal DNA uptake

J Bacteriol. 1998 Feb;180(3):746-8. doi: 10.1128/JB.180.3.746-748.1998.

Abstract

Haemophilus influenzae Rd is a gram-negative natural transformer. A mutant strain, RJ248, that has normal DNA uptake and translocation but whose transformation frequency is 300 times lower than that of wild-type H. influenzae and whose phage recombination is 8 times lower was isolated. The affected gene, comM, is induced during competence development in wild-type H. influenzae but not in RJ248.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • DNA Transposable Elements*
  • Haemophilus influenzae / genetics*
  • Haemophilus influenzae / growth & development
  • Mutagenesis, Insertional
  • Promoter Regions, Genetic
  • Transformation, Bacterial*

Substances

  • DNA Transposable Elements