Abstract
We cloned the myo3+ gene of Schizosaccharomyces pombe which encodes a type-II myosin heavy chain. myo3 null cells showed a defect in cytokinesis under certain conditions. Overproduction of Myo3 also showed a defect in cytokinesis. Double mutant analysis indicated that Myo3 genetically interacts with Cdc8 tropomyosin and actin. Myo3 may be implicated in cytokinesis and stabilization of F-actin cables. Moreover, the function of Myo2 can be replaced by overexpressed Myo3. We observed a modest synthetic interaction between Myo2 and Myo3. Thus, Myo2 and Myo3 seem to cooperate in the formation of the F-actin ring in S. pombe.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Actins / metabolism
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Amino Acid Sequence
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Bacterial Proteins / metabolism
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Blotting, Southern
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Carrier Proteins / genetics
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Carrier Proteins / metabolism*
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Cell Division / physiology
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Cell Nucleus
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Cloning, Molecular
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Fluorescent Dyes / metabolism
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Fungal Proteins / chemistry
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Fungal Proteins / metabolism
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Gene Expression Regulation, Fungal / genetics
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Microscopy, Fluorescence
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Molecular Sequence Data
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Myosin Heavy Chains / chemistry*
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Myosin Heavy Chains / metabolism
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Myosin Type II*
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Myosin Type V*
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Myosins / chemistry*
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Myosins / metabolism
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Phenotype
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Restriction Mapping
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Saccharomyces cerevisiae Proteins*
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Schizosaccharomyces / chemistry*
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Schizosaccharomyces / genetics
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Schizosaccharomyces pombe Proteins*
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Sequence Analysis, DNA
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Tropomyosin / metabolism
Substances
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Actins
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Bacterial Proteins
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Carrier Proteins
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Fluorescent Dyes
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Fungal Proteins
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MYO2 protein, S cerevisiae
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MYO2 protein, S pombe
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Myp2 protein, S pombe
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Saccharomyces cerevisiae Proteins
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Schizosaccharomyces pombe Proteins
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Tropomyosin
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Myosin Type II
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Myosin Type V
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Myosin Heavy Chains
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Myosins