Abstract
A new inducible yeast expression vector, pXS7, was constructed by using the promoter and terminator sequences from the Saccharomyces cerevisiae SOR1 gene, which codes for the sorbitol dehydrogenase protein. We cloned the coding sequence of the Saccharomyces YEF3 gene in this vector and demonstrated an increase in YEF3 protein levels when cells were grown in the presence of the sugar sorbitol.
MeSH terms
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Base Sequence
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Fungal Proteins*
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Genetic Vectors*
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L-Iditol 2-Dehydrogenase / genetics*
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Molecular Sequence Data
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Peptide Elongation Factors / biosynthesis*
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Recombinant Proteins / biosynthesis*
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Saccharomyces cerevisiae / genetics*
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Saccharomyces cerevisiae Proteins
Substances
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Fungal Proteins
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Peptide Elongation Factors
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Recombinant Proteins
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Saccharomyces cerevisiae Proteins
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YEF3 protein, S cerevisiae
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L-Iditol 2-Dehydrogenase