Abstract
In mice, Pax5 gene is indispensable for B cell development. Pax5-deficient mice fail to produce mature B cells owing to complete arrest of B cell development at a precursor stage. However, the lineage and stage of human Pax5 gene expression have remained elusive. In this investigation expression of the human Pax5 gene was studied. Pax5 gene expression was detected in B cell lines but not in myeloma cell lines. CD19 expression was correlated with Pax5 gene expression. Adult spleen and bone marrow and fetal spleen and liver showed strong Pax5 gene expression, as did the corresponding mouse tissues, as reported previously. In common variable immunodeficiency (CVID) peripheral blood lymphocytes (PBL) with a decreased number of B cells, no Pax5 gene expression was detected. Some CVID PBL stimulated with IL-2, IL-10 and anti-CD40 monoclonal antibody, expressed the Pax5 gene. Defect of Pax5 gene expression in CVID may be caused by regulatory T cell disorder.
MeSH terms
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Adolescent
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Adult
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Antibodies, Monoclonal / pharmacology
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B-Lymphocytes / drug effects
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B-Lymphocytes / metabolism
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CD40 Antigens / immunology
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Cell Differentiation / physiology
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Cell Line
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Child
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Child, Preschool
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Common Variable Immunodeficiency / blood
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Common Variable Immunodeficiency / genetics
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Common Variable Immunodeficiency / metabolism*
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DNA-Binding Proteins / biosynthesis*
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / physiology
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Female
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Gene Expression
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Gene Expression Regulation
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Hematopoietic Stem Cells / metabolism*
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Humans
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Interleukin-10 / pharmacology
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Interleukin-2 / pharmacology
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Lymphocyte Activation / drug effects
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Male
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Nuclear Proteins / biosynthesis*
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Nuclear Proteins / genetics
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Nuclear Proteins / physiology
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PAX5 Transcription Factor
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Polymerase Chain Reaction
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Stimulation, Chemical
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Transcription Factors*
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Transcription, Genetic
Substances
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Antibodies, Monoclonal
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CD40 Antigens
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DNA-Binding Proteins
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Interleukin-2
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Nuclear Proteins
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PAX5 Transcription Factor
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PAX5 protein, human
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Transcription Factors
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Interleukin-10