Abstract
Leishmania infantum immunoelectrophoretic antigen 24 (AG 24), a visceral leishmaniasis associated immunodominant antigen, has been characterized with a monospecific antiserum by combining SDS-PAGE, immunoblotting, metabolic labelling, radio-immunoprecipitation and in vitro poly A+ mRNA translation. AG 24 appeared to correspond to a multi-antigen family of 6-9 members ranging from 20 to 31 kDa and proteinic by nature with no post-translational modifications. A similar banding pattern was recognized by infection sera. AG 24 was not found exposed on the cell surface.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antigens, Protozoan / analysis*
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Antigens, Protozoan / chemistry
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Antigens, Protozoan / genetics
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Antigens, Protozoan / immunology
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Electrophoresis, Polyacrylamide Gel
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Fluorescent Antibody Technique, Indirect
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Humans
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Immune Sera / immunology
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Immunoblotting / methods
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Immunodominant Epitopes / analysis*
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Immunodominant Epitopes / chemistry
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Immunodominant Epitopes / genetics
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Immunodominant Epitopes / immunology
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Immunoelectrophoresis / methods
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Leishmania infantum / immunology*
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Leishmaniasis, Visceral / diagnosis
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Leishmaniasis, Visceral / immunology
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Molecular Structure
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Protein Biosynthesis
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RNA, Messenger / genetics
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RNA, Protozoan / genetics
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Radioimmunoprecipitation Assay
Substances
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Antigens, Protozoan
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Immune Sera
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Immunodominant Epitopes
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RNA, Messenger
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RNA, Protozoan