Abstract
The genomic DNA encoding bovine Thyrotropin-Releasing Hormone Receptor (TRHR) was isolated from a bovine (Holstein) genomic library. Using PCR fragments of bovine candidate TRHR Transmembrane domain (TMD-III-IV) and C-terminus domain of mouse TRHR cDNA as probes, 9 x 10(5) plaques were screened to obtain several clones each containing the N-terminus or C-terminus domain. The bovine TRHR gene encoded 398 amino acids and has a long intron. The identity of the deduced amino acid sequence of bovine TRHR exceeded 88% that of mouse, rat or human. RT-PCR analysis indicated TRHR mRNA to be expressed in the pituitary and brain.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Cattle
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Cell Membrane / metabolism
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Cloning, Molecular
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DNA Probes
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DNA, Complementary
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Genomic Library
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Humans
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Introns
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Mice
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Molecular Sequence Data
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Open Reading Frames
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Organ Specificity
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Polymerase Chain Reaction
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RNA, Messenger / biosynthesis
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Rats
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Receptors, Thyrotropin-Releasing Hormone / biosynthesis*
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Receptors, Thyrotropin-Releasing Hormone / genetics*
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Sequence Alignment
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Sequence Homology, Amino Acid
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Transcription, Genetic
Substances
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DNA Probes
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DNA, Complementary
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RNA, Messenger
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Receptors, Thyrotropin-Releasing Hormone
Associated data
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GENBANK/AB001751
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GENBANK/AB001752
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GENBANK/D83964