Sequence-independent method for in vitro generation of nested deletions for sequencing large DNA fragments

Anal Biochem. 1998 Apr 10;258(1):138-41. doi: 10.1006/abio.1997.2591.

Authors

A F Fradkov¹, K A Lukyanov, M V Matz, L B Diatchenko, P D Siebert, S A Lukyanov

Affiliation

¹ Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Science, Miklukho-Maklaya 16/10, V-437, Moscow, 117871, Russia.

PMID: 9527860
DOI: 10.1006/abio.1997.2591

No abstract available

MeSH terms

Bacteriophage T7 / genetics
Base Sequence
Cloning, Molecular / methods
DNA / genetics*
DNA / isolation & purification
DNA Primers / genetics
Deoxyribonuclease I
Polymerase Chain Reaction / methods
Promoter Regions, Genetic
Sequence Analysis, DNA / methods*
Sequence Deletion*

Substances

DNA Primers
DNA
Deoxyribonuclease I