Abstract
Phage library clones selected by a conformational epitope-recognizing and inhibitory monoclonal antibody may display moieties that mimic a receptor/ligand-like three-dimensional structure. This pseudoreceptor/ligand should be able to bind to natural ligand/receptor molecules. We tested this idea using anti-T cell costimulatory molecule antibodies and successfully isolated phage clones with costimulatory effects on T-cell proliferation. This strategy facilitates the designing of regulatory peptide molecules in the absence of precise information about the structure-function relationships in receptor/ligand interactions.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Abatacept
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Amino Acid Sequence
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Animals
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Antibodies, Monoclonal
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Antigens, CD / immunology
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Antigens, CD / metabolism
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Antigens, Differentiation / immunology*
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B7-1 Antigen / immunology
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B7-1 Antigen / metabolism
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B7-2 Antigen
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Bacteriophages / genetics
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Bacteriophages / immunology
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CTLA-4 Antigen
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Cell Division / drug effects
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Cricetinae
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Epitopes
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Immunoconjugates*
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Membrane Glycoproteins / immunology
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Membrane Glycoproteins / metabolism
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Mice
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Mice, Inbred BALB C
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Models, Immunological
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Molecular Sequence Data
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Peptides / immunology*
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Peptides / metabolism*
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Peptides / pharmacology
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T-Lymphocytes / drug effects
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T-Lymphocytes / immunology*
Substances
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Antibodies, Monoclonal
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Antigens, CD
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Antigens, Differentiation
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B7-1 Antigen
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B7-2 Antigen
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CTLA-4 Antigen
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Cd86 protein, mouse
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Ctla4 protein, mouse
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Epitopes
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Immunoconjugates
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Membrane Glycoproteins
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Peptides
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Abatacept