Objective: To investigate the mutation of human K-ras oncogene in the blood of patients with lung cancer.
Methods: PCR and sequencing methods were used to analyse the exon 1. Twelve patients'blood samples and 31 normals' blood samples were detected.
Results: In all 43 samples, the K-ras codon 11 was CCT (proline), and the opposite strand sequenced was AGG. It was different from the standard GCT(Alanine) sequenced by foreign authors.
Conclusion: The 11th codon of the human K-ras oncogene in Chinese is CCT (Proline). As the K-ras codon 12 is a hot spot, this finding may be of importance to clinical genetic diagnosis.