Abstract
Body cavity-based lymphoma, also known as primary effusion lymphoma, is a newly recognized acquired immunodeficiency syndrome (AIDS)-related lymphoma that has been linked to the Kaposi's sarcoma-associated herpesvirus (KSHV/HHV-8). To date, direct visualization of the virus in a clinical sample has not been demonstrated. We have performed an extensive clinical, histologic, immunophenotypic, ultrastructural, and molecular genetic correlative study on multiple tissue samples obtained premortem and at autopsy from an patient with AIDS with Kaposi's sarcoma and body cavity-based lymphomas. We demonstrate the presence of human herpesvirus-8 in a primary clinical sample at the ultrastructural and molecular level, as well as document multiple lymphomatous tumor masses at autopsy.
MeSH terms
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Adult
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Cytomegalovirus / isolation & purification
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DNA Nucleotidyltransferases / analysis
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DNA, Viral / analysis
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Fatal Outcome
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HIV Infections / complications
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Herpesvirus 4, Human / isolation & purification*
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Herpesvirus 8, Human / isolation & purification*
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Humans
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Immunohistochemistry
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In Situ Hybridization
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Lymphoma, AIDS-Related / chemistry
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Lymphoma, AIDS-Related / complications
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Lymphoma, AIDS-Related / pathology*
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Lymphoma, AIDS-Related / ultrastructure
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Lymphoma, AIDS-Related / virology*
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Male
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Microscopy, Electron
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Polymerase Chain Reaction
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Proto-Oncogene Proteins c-bcl-2 / analysis
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RNA, Messenger / analysis
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RNA, Viral / analysis
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Sarcoma, Kaposi / complications
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Sarcoma, Kaposi / virology*
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Skin Neoplasms / complications
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Skin Neoplasms / virology*
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VDJ Recombinases
Substances
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DNA, Viral
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Epstein-Barr virus encoded RNA 1
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Epstein-Barr virus encoded RNA 2
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Proto-Oncogene Proteins c-bcl-2
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RNA, Messenger
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RNA, Viral
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DNA Nucleotidyltransferases
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VDJ Recombinases