Abstract
The NF2 tumor suppressor gene product, designated merlin, belongs to the family of molecules that links membranous protein with the cytoskeleton. We have previously shown that merlin was co-immunoprecipitated with a cellular protein, p85, in cultured cell. To analyze the alteration of merlin and associated proteins in surgical specimens, we developed a new method for biotin-labeling of whole cellular proteins. Screening of tumor tissues using our method showed that none of malignant gliomas and half of the NF2-related tumors had altered p85 and merlin. Our detection method seems useful for the screening of merlin alterations in NF2-related tumors.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Avian Proteins*
-
Brain Neoplasms / genetics
-
Brain Neoplasms / metabolism*
-
Brain Neoplasms / pathology
-
Cloning, Molecular
-
Cytoskeletal Proteins / isolation & purification
-
Cytoskeletal Proteins / metabolism*
-
Genes, Neurofibromatosis 2*
-
Glioma / genetics
-
Glioma / metabolism*
-
Glioma / pathology
-
Humans
-
Membrane Proteins / biosynthesis
-
Membrane Proteins / isolation & purification
-
Membrane Proteins / metabolism*
-
Meningeal Neoplasms / genetics
-
Meningeal Neoplasms / metabolism
-
Meningeal Neoplasms / pathology
-
Meningioma / genetics
-
Meningioma / metabolism
-
Meningioma / pathology
-
Neurilemmoma / genetics
-
Neurilemmoma / metabolism
-
Neurilemmoma / pathology
-
Neurofibromin 2
-
Polymerase Chain Reaction
-
Recombinant Fusion Proteins / biosynthesis
-
Recombinant Fusion Proteins / isolation & purification
-
Recombinant Fusion Proteins / metabolism
-
Tumor Cells, Cultured
Substances
-
Avian Proteins
-
Cytoskeletal Proteins
-
Membrane Proteins
-
Neurofibromin 2
-
Recombinant Fusion Proteins
-
cytoskeletal p85 protein, chicken