Abstract
In rheumatoid arthritis (RA) cell proliferation and altered metalloproteinase expression of synovial lining cells are associated with increased levels of TNF-alpha in the rheumatoid joint. We previously showed that synoviocytes of RA patients express high levels of the transcription factor Egr-1. Here we report that TNF-alpha is capable of inducing high Egr-1 mRNA levels in human skin fibroblasts and in synoviocytes from both, RA and reactive arthritis patients. Moreover, we observed in vitro a marked increase in fibroblast proliferation, a loss of growth inhibition by cell-to-cell contact with pannus-like cell growth and an altered cytokine expression pattern when synoviocytes were cultured in presence of TNF-alpha.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Cell Division / drug effects
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Cells, Cultured
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Cytokines / drug effects*
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Cytokines / genetics
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DNA-Binding Proteins / drug effects*
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DNA-Binding Proteins / genetics
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Early Growth Response Protein 1
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Fibroblasts / cytology
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Fibroblasts / drug effects
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Gene Expression / drug effects
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Gene Expression / genetics
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Humans
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Immediate-Early Proteins*
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Male
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Skin / cytology
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Skin / drug effects
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Synovial Membrane / chemistry
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Synovial Membrane / cytology
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Synovial Membrane / drug effects*
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Transcription Factors / drug effects*
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Transcription Factors / genetics
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Transcription, Genetic / drug effects
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Transcription, Genetic / genetics
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Tumor Necrosis Factor-alpha / pharmacology*
Substances
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Cytokines
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DNA-Binding Proteins
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EGR1 protein, human
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Early Growth Response Protein 1
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Immediate-Early Proteins
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Transcription Factors
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Tumor Necrosis Factor-alpha