The effect of IL-12 treatment on vaccine-enhanced illness during infection with respiratory syncytial virus

Dev Biol Stand. 1998:92:179-85.

Abstract

In humans and mice, sensitisation to respiratory syncytial virus (RSV) antigens can result in severe inflammatory lung disease during subsequent infection with the virus. Although specific antiviral T cells are thought to be responsible for this augmentation of disease, the precise role of different functional subsets is unknown. BALB/c mice sensitised to the major surface glycoprotein (G) of RSV expressed by recombinant vaccinia virus develop Th2-driven lung eosinophilia after intranasal challenge with the virus. Mice treated with IL-12 at various times during vaccination and challenge, had reduced vaccine-induced lung eosinophilia but increased total pulmonary lymphoid cell infiltration. Intracellular cytokine analysis showed that interferon-gamma production during challenge was increased and IL-4 and IL-5 reduced by IL-12 treatment. Though IL-12 treatment reduced lung eosinophilia, illness (as assessed by weight loss) was not eliminated and sometimes increased. Reversing Th2-associated pathology with IL-12 does not necessarily benefit the host.

Publication types

  • Review

MeSH terms

  • Adjuvants, Immunologic / therapeutic use*
  • Animals
  • Bronchiolitis, Viral / complications
  • Bronchiolitis, Viral / immunology*
  • Bronchiolitis, Viral / prevention & control
  • Child
  • Disease Models, Animal
  • HN Protein*
  • Humans
  • Hypersensitivity, Immediate / etiology
  • Interleukin-12 / therapeutic use*
  • Respiratory Syncytial Virus Infections / complications
  • Respiratory Syncytial Virus Infections / immunology*
  • Respiratory Syncytial Virus Infections / prevention & control
  • Respiratory Syncytial Viruses
  • Viral Envelope Proteins
  • Viral Proteins / immunology
  • Viral Vaccines / adverse effects*
  • Viral Vaccines / immunology*

Substances

  • Adjuvants, Immunologic
  • HN Protein
  • Viral Envelope Proteins
  • Viral Proteins
  • Viral Vaccines
  • attachment protein G
  • Interleukin-12