Abstract
The human immunodeficiency virus type 1 capsid protein contains a conserved P217X4PX2PX5P231 motif. Mutation at Pro-222 decreases virion incorporation of cyclophilin A, while mutation at Pro-231 abolishes infectivity. Although viral RNA incorporation and protease cleavage of the Gag precursor were not affected by these mutations, cryoelectron microscopy revealed a loss of virion maturation in P231A particles.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Binding Sites
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Cell Line
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Endopeptidases / metabolism
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Gene Products, gag / metabolism
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HIV-1 / genetics*
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HIV-1 / metabolism
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HIV-1 / physiology
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HIV-1 / ultrastructure*
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Humans
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Microscopy, Electron
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Mutagenesis, Site-Directed*
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Peptidylprolyl Isomerase / metabolism*
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Virion / ultrastructure
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Virus Replication
Substances
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Gene Products, gag
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Endopeptidases
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Peptidylprolyl Isomerase