Under suspicion on an injection of high doses of insulin with suicidal or homicidal intention, the local detection of a vital injection of insulin is necessary for the critical examination. The submitted immunohistochemical method shows, that: the insulin-specific positive immunoreaction of the subcutaneous fatty tissue is assigned to an injection of insulin, although a missing staining does not exclude a premortal application of insulin, a widened immunopositive intercellular space between the subcutaneous lipocytes as expression of a local edema points to the intravitality of the injection of insulin.