The regional excision of parotid gland (resection of the tumor with its surrounding glands) was applied in 54 cases with Warthin tumor. Its role was evaluated by follow-up study, iodine-starch test (Minor test), radionuclide scintigraphy, and comparison with superficial parotidectomy. The results showed that regional excision had the following advantages: shortening the time of operation, decreasing the injury of facial nerve, avoiding the facial deformity and Frey syndrome, and reserving the function of parotid glands. The theoretical basis, indications, and some considerable questions during the operation were discussed.