Testing of toxic and DNA-damaging effects of N-cyclohexylthiophthalimide (Duslin P) on hamster V79 cells

Folia Biol (Praha). 1997;43(6):239-42.

Abstract

N-cyclohexylthiophthalimide, commercial name Duslin P, at concentrations 0.5-2 micrograms/ml inhibited proliferation of V79 cells and at concentrations > 2 micrograms/ml acted cytotoxically. Inhibition of cumulative DNA synthesis correlated well with the deleterious effects of Duslin P on growth activity and plating efficiency. DNA synthesis was not renewed even 6 h after the treatment of cells. Alkaline elution of DNA of V79 cells treated with Duslin P did not confirm our expectation that this chemical compound has a DNA-damaging effect. Duslin P strongly inhibited protein synthesis at concentrations > 2 micrograms/ml. We suggest that the cytotoxic effects of Duslin P are not accompanied by any genotoxic effects.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line
  • Cell Survival / drug effects
  • Cricetinae
  • Cricetulus
  • DNA / drug effects*
  • DNA Damage*
  • Nucleic Acid Synthesis Inhibitors / toxicity*
  • Phthalimides / toxicity*
  • Protein Synthesis Inhibitors / toxicity*

Substances

  • Nucleic Acid Synthesis Inhibitors
  • Phthalimides
  • Protein Synthesis Inhibitors
  • N-(cyclohexylthio)phthalimide
  • DNA