Abstract
Using a two-dimensional DNA mapping method, we detected four NotI restriction sites that escape chromosome X-specific methylation in humans. Two genes corresponding to two of these sites that lie in the region of Xp11.2-p11.4 were cloned and their properties studied. One of the genes matched a known gene, but the other, termed EXLM1, is novel and is predominantly expressed in cultured lymphocytes and skeletal muscle.
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Blotting, Southern
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Cell Line, Transformed
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Cloning, Molecular
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DNA Methylation
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DNA, Complementary / isolation & purification
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Deoxyribonucleases, Type II Site-Specific / metabolism
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Dosage Compensation, Genetic*
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Electrophoresis, Gel, Two-Dimensional
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Humans
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Mediator Complex
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Molecular Sequence Data
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Organ Specificity
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Restriction Mapping / methods*
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Sequence Analysis, DNA
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Trans-Activators*
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Transcription, Genetic
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X Chromosome / genetics*
Substances
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DNA, Complementary
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MED14 protein, human
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Mediator Complex
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Trans-Activators
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Deoxyribonucleases, Type II Site-Specific
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GCGGCCGC-specific type II deoxyribonucleases
Associated data
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GENBANK/AB006651
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GENBANK/AB006652