Nitric oxide-mediated cGMP production in the islands of Calleja in the rat

Brain Res. 1998 Apr 6;789(1):175-8. doi: 10.1016/s0006-8993(98)00099-7.

Abstract

cGMP-immunostaining in the islands of Calleja (ICj) in slices incubated in vitro partially co-localized with nitric oxide synthase (NOS) inside the ICj. No cGMP-immunostaining was found outside the ICj in unstimulated slices, whereas the NO-donor sodium nitroprusside (SNP) stimulated cGMP in cells and fibers bordering on the ICj. These findings show an ongoing NO synthesis in in vitro slices and suggest a relatively restricted diffusion range for endogenously synthesized NO.

MeSH terms

  • Animals
  • Cyclic GMP / antagonists & inhibitors
  • Cyclic GMP / biosynthesis*
  • Enzyme Inhibitors / pharmacology
  • Guanylate Cyclase / antagonists & inhibitors
  • Male
  • NG-Nitroarginine Methyl Ester / pharmacology
  • Nitric Oxide / physiology*
  • Nitroprusside / pharmacology
  • Olfactory Bulb / metabolism*
  • Oxadiazoles / pharmacology
  • Quinoxalines / pharmacology
  • Rats
  • Rats, Inbred Lew
  • Tissue Distribution

Substances

  • 1H-(1,2,4)oxadiazolo(4,3-a)quinoxalin-1-one
  • Enzyme Inhibitors
  • Oxadiazoles
  • Quinoxalines
  • Nitroprusside
  • Nitric Oxide
  • Guanylate Cyclase
  • Cyclic GMP
  • NG-Nitroarginine Methyl Ester