Cytotoxicity of endodontic materials

J Endod. 1998 Feb;24(2):91-6. doi: 10.1016/S0099-2399(98)80084-8.

Abstract

An in vitro cell culture model of human gingival fibroblasts and L-929 cells was used to measure the cytotoxicity of currently used root canal sealers Endomet, CRCS, and AH26 and root-end filling materials Amalgam, Gallium GF2, Ketac Silver, mineral trioxide aggregate (MTA), and Super-EBA. Cytotoxic effects were assessed using the MTT assay for mitochondrial enzyme activity and the CV assay for cell numbers. Using inserts culture and L-929 fibroblasts. All-Bond-2 was also evaluated. The statistical analysis of results showed that CRCS was the least cytotoxic sealer followed by Endomet and AH26. Among root-end filling materials, MTA was not cytotoxic; Gallium GF2 displayed little cytotoxicity; and Ketac Silver, Super-EBA, and Amalgam showed higher levels of cytotoxicity. All Bond-2 also displayed a high degree of cytotoxicity. CRCS was the best root canal sealer and MTA the best root-end filling material. The outcome was favorable also for Gallium GF2 as a retrofilling material.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Alloys / toxicity
  • Aluminum Compounds / toxicity
  • Analysis of Variance
  • Animals
  • Bismuth / toxicity
  • Calcium Compounds / toxicity
  • Calcium Hydroxide / toxicity
  • Cells, Cultured
  • Cermet Cements / toxicity
  • Dental Alloys / toxicity
  • Dentin-Bonding Agents / toxicity
  • Drug Combinations
  • Epoxy Resins*
  • Evaluation Studies as Topic
  • Fibroblasts / drug effects*
  • Gallium / toxicity
  • Gingiva / cytology
  • Gingiva / drug effects*
  • Humans
  • L Cells
  • Methacrylates / toxicity
  • Methenamine / toxicity
  • Mice
  • Oxides / toxicity
  • Retrograde Obturation
  • Root Canal Filling Materials / toxicity*
  • Silicates / toxicity
  • Silver / toxicity
  • Titanium / toxicity
  • Zinc Oxide / toxicity
  • Zinc Oxide-Eugenol Cement / toxicity

Substances

  • Alloys
  • Aluminum Compounds
  • Calcium Compounds
  • Cermet Cements
  • Dental Alloys
  • Dentin-Bonding Agents
  • Drug Combinations
  • Endomet
  • Epoxy Resins
  • Methacrylates
  • Oxides
  • Root Canal Filling Materials
  • Silicates
  • Zinc Oxide-Eugenol Cement
  • mineral trioxide aggregate
  • Calcibiotic Root Canal Sealer
  • gallium alloy GF
  • All-Bond 2
  • Silver
  • epoxy resin AH-26
  • Super EBA
  • Valiant PhD
  • Gallium
  • Titanium
  • Methenamine
  • Calcium Hydroxide
  • Zinc Oxide
  • Bismuth