Abstract
Several members of the Ly-49 receptor family inhibit NK cell-mediated lysis of targets expressing appropriate MHC class I molecules. Ly-49D and Ly-49H, two Ly-49 molecules that lack immunoreceptor tyrosine-based inhibitory motifs (ITIM) in their cytoplasmic domains, associate with mouse DAP12, a molecule that possesses an immunoreceptor tyrosine-based activation motif (ITAM). Cotransfection of either Ly-49D or Ly-49H with DAP12 induces surface expression of both Ly-49 and DAP12. The Ly-49/DAP12 complex was coimmunoprecipitated from the transfected cells, demonstrating a physical association of DAP12 with Ly-49D or Ly-49H in the plasma membrane. Stimulation of transfectants with Abs recognizing either Ly-49D or Ly-49H results in cellular activation, as assessed by induction of tyrosine phosphorylation of multiple cellular substrates.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adaptor Proteins, Signal Transducing
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Animals
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Antigens, Ly*
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Antigens, Surface / metabolism*
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Interleukin-2 / pharmacology
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Killer Cells, Natural / immunology
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Killer Cells, Natural / metabolism*
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Lectins, C-Type
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Lymphocyte Activation
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Membrane Glycoproteins / genetics
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Membrane Glycoproteins / metabolism*
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Membrane Proteins
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Mice
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Receptors, Amino Acid / immunology
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Receptors, Immunologic / metabolism*
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Receptors, Immunologic / physiology*
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Receptors, NK Cell Lectin-Like
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Transcription, Genetic / immunology
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Transfection / immunology
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Tyrosine / metabolism
Substances
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Adaptor Proteins, Signal Transducing
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Antigens, Ly
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Antigens, Surface
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Interleukin-2
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Lectins, C-Type
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Membrane Glycoproteins
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Membrane Proteins
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Receptors, Amino Acid
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Receptors, Immunologic
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Receptors, NK Cell Lectin-Like
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TYROBP protein, human
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tyrosine receptor
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Tyrosine