beta-Glucosylation of chitooligomers by galactosyltransferase

Carbohydr Res. 1997 Dec;305(3-4):517-23. doi: 10.1016/s0008-6215(97)00248-6.

Abstract

Galactosyltransferase from bovine milk was found to be able to utilise UDP-Glc to transfer Glc onto GlcNAc and chitooligomers[-beta-GlcNAc-(1-->4)-]n, n = 2-4. beta-Glucosylated products were used in binding studies with NKR-P1A protein cloned from rat natural killer cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetylglucosamine / chemistry*
  • Animals
  • Antigens, Surface / metabolism*
  • Binding, Competitive
  • Carbohydrate Sequence
  • Cattle
  • Galactosyltransferases / metabolism*
  • Glucosides / chemical synthesis*
  • Killer Cells, Natural / chemistry
  • Lectins, C-Type*
  • Magnetic Resonance Spectroscopy
  • Milk / enzymology
  • Molecular Sequence Data
  • NK Cell Lectin-Like Receptor Subfamily B
  • Oligosaccharides / chemical synthesis
  • Protein Binding / physiology
  • Rats
  • Uridine Diphosphate Glucose / metabolism

Substances

  • Antigens, Surface
  • Glucosides
  • Lectins, C-Type
  • NK Cell Lectin-Like Receptor Subfamily B
  • Oligosaccharides
  • Galactosyltransferases
  • Uridine Diphosphate Glucose
  • Acetylglucosamine