Comparison of sample preparation methods for detection of Legionella pneumophila in culture-positive bronchoalveolar lavage fluids by PCR

B Jaulhac; M Reyrolle; Y K Sodahlon; S Jarraud; M Kubina; H Monteil; Y Piémont; J Etienne

doi:10.1128/JCM.36.7.2120-2122.1998

Comparison of sample preparation methods for detection of Legionella pneumophila in culture-positive bronchoalveolar lavage fluids by PCR

J Clin Microbiol. 1998 Jul;36(7):2120-2. doi: 10.1128/JCM.36.7.2120-2122.1998.

Authors

B Jaulhac¹, M Reyrolle, Y K Sodahlon, S Jarraud, M Kubina, H Monteil, Y Piémont, J Etienne

Affiliation

¹ Institut de Bactériologie de la Faculté de Médecine, Université Louis Pasteur et Hopitaux Universitaires de Strasbourg, France. [email protected]

Abstract

A prospective study was conducted on 25 Legionella pneumophila culture-positive and 98 culture-negative bronchoalveolar lavage fluid samples to compare two DNA preparation methods: a rapid modified Chelex-based protocol and a proteinase K method. PCR was found to be more sensitive with the Chelex-based method (P = 0.03). N difference was found concerning the inhibition rate.

Publication types

Comparative Study

MeSH terms

Bacteriological Techniques
Bronchoalveolar Lavage Fluid / microbiology*
DNA, Bacterial / isolation & purification*
Endopeptidase K
Humans
Legionella pneumophila / isolation & purification*
Polymerase Chain Reaction / methods*
Polystyrenes
Polyvinyls
Prospective Studies
Sensitivity and Specificity

Substances

DNA, Bacterial
Polystyrenes
Polyvinyls
chelex
Endopeptidase K