The temperature distribution within a wide-gap (3 mm) continuous free-flow electrophoresis (CFFE) chamber was investigated by direct measurements. The walls of the chambers consisted of Plexiglas and thermocouples were used as temperature sensors. The study of different operating and heat dissipating conditions revealed that lateral temperature gradients arise in CFFE chambers in addition to an axial increase in temperature of 0.25 degrees C per Watt of electric power and per minute of residence time in the absence of cooling.