Competitive PCR for quantification of BM5d proviral DNA in mice with AIDS

J Clin Microbiol. 1998 Aug;36(8):2371-4. doi: 10.1128/JCM.36.8.2371-2374.1998.

Abstract

Murine AIDS in C57BL/6 mice is caused by a unique mixture of murine leukemia viruses. We report the use of a competitive PCR to detect and quantitate BM5d proviral DNA. This assay allowed discrimination among endogenous wild-type murine retroviruses and BM5d sequences. Furthermore, the method was subsequently used to evaluate the amount of BM5d in infected mice and in infected AZT (zidovudine)-treated mice, providing an effective way to quantitatively evaluate drug efficacy in the murine AIDS model.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • DNA, Viral / analysis*
  • Gene Dosage
  • Leukemia Virus, Murine / genetics*
  • Lymph Nodes / virology
  • Mice
  • Mice, Inbred C57BL
  • Murine Acquired Immunodeficiency Syndrome / drug therapy
  • Murine Acquired Immunodeficiency Syndrome / virology*
  • Polymerase Chain Reaction / methods*
  • Proviruses / genetics*
  • Zidovudine / therapeutic use

Substances

  • DNA, Viral
  • Zidovudine