Abstract
We describe a new mature B-cell acute lymphoblastic leukemia (ALL) cell line designated Z-138 that was derived from a patient with chronic lymphocytic leukemia (CLL) whose disease underwent transformation to a rare, aggressive form of mature B-cell ALL. This cell line has an L3 morphology, ultrastructural characteristics of lymphoblasts, B-lineage surface markers and an immunoglobulin heavy-chain gene rearrangement identical to the rearrangement observed in the patient's blasts from whom the cell line was derived. Z-138 cells produce granulocyte-macrophage colony-stimulating factor (GM-CSF) and high levels of granulocyte-CSF (G-CSF), but they do not exhibit a proliferative response to either cytokine. Both the patient's lymphoblasts and Z-138 cells exhibited cytogenetic abnormalities including t(8;14), t(14;18) and a chromosome 11 abnormality similar to the t(11;14) of the parental cells, resulting in marked overexpression of cyclin D1 (BCL-1 (PRAD1)) mRNA in Z-138 cells. Since these karyotypic anomalies have been associated with low grade (t(14;18)), intermediate grade (t(11;14)) and high grade (t(8;14)) lymphomas, their development may be involved in the unusual aggressive transformation of this patient's CLL.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Aged
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Blotting, Southern
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Bone Marrow Cells / pathology
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Burkitt Lymphoma / etiology*
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Burkitt Lymphoma / immunology
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Burkitt Lymphoma / pathology*
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Cell Transformation, Neoplastic / pathology*
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Cell Transformation, Viral
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Chromosome Aberrations / genetics*
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Chromosomes, Human, Pair 9 / genetics*
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Clone Cells / chemistry
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DNA / analysis
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Fusion Proteins, bcr-abl / biosynthesis
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Gene Rearrangement, B-Lymphocyte, Heavy Chain / genetics
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Granulocyte Colony-Stimulating Factor / biosynthesis
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Granulocyte Colony-Stimulating Factor / pharmacology
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Granulocyte-Macrophage Colony-Stimulating Factor / biosynthesis
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Granulocyte-Macrophage Colony-Stimulating Factor / pharmacology
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Herpesvirus 4, Human / isolation & purification
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Humans
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Immunoglobulin J-Chains / genetics
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Immunophenotyping
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Interleukin-1 / biosynthesis
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Interleukin-6 / biosynthesis
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Karyotyping
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Leukemia, Lymphocytic, Chronic, B-Cell / pathology*
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Lymphocyte Activation / drug effects
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Male
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Microscopy, Electron
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RNA / analysis
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Transforming Growth Factor beta / biosynthesis
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Tumor Cells, Cultured / cytology*
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Tumor Cells, Cultured / physiology
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Tumor Cells, Cultured / virology
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Tumor Necrosis Factor-alpha / biosynthesis
Substances
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Immunoglobulin J-Chains
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Interleukin-1
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Interleukin-6
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Transforming Growth Factor beta
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Tumor Necrosis Factor-alpha
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Granulocyte Colony-Stimulating Factor
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RNA
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Granulocyte-Macrophage Colony-Stimulating Factor
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DNA
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Fusion Proteins, bcr-abl