Abstract
NK recognition and lysis of targets are mediated by activation receptor(s) whose effects may be over-ridden by inhibitory receptors recognizing class I MHC on the target. Incubation of normal lymphoblasts with a peptide that can bind to their class I MHC renders them sensitive to lysis by syngeneic NK cells. By binding to class I MHC, the peptide alters or masks the target structure recognized by an inhibitory NK receptor(s). This target structure is most likely an "empty" dimer of class I heavy chain and beta2m as opposed to a "full" class I trimer formed by binding of specific peptide that is recognized by CTL.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Anti-Bacterial Agents / pharmacology
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Antigens, Ly*
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Antigens, Surface / metabolism
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Brefeldin A
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Concanavalin A / pharmacology
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Cyclopentanes / pharmacology
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Cytotoxicity Tests, Immunologic
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Cytotoxicity, Immunologic / drug effects
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H-2 Antigens / metabolism
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Histocompatibility Antigens Class I / biosynthesis
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Histocompatibility Antigens Class I / drug effects
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Histocompatibility Antigens Class I / metabolism*
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Killer Cells, Natural / drug effects
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Killer Cells, Natural / immunology*
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Killer Cells, Natural / metabolism
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Lectins, C-Type
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Lymphocyte Activation
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Macrolides
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Membrane Glycoproteins / metabolism
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Mice
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Mice, Inbred BALB C
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Mice, Inbred C57BL
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Mice, Knockout
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Mice, Nude
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Molecular Sequence Data
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Peptide Fragments / immunology
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Peptide Fragments / metabolism
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Protein Binding / immunology
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Protein Synthesis Inhibitors / pharmacology
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Receptors, Immunologic / immunology
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Receptors, KIR
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Receptors, NK Cell Lectin-Like
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T-Lymphocytes, Cytotoxic / immunology
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Time Factors
Substances
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Anti-Bacterial Agents
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Antigens, Ly
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Antigens, Surface
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Cyclopentanes
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H-2 Antigens
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Histocompatibility Antigens Class I
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Lectins, C-Type
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Macrolides
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Membrane Glycoproteins
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Peptide Fragments
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Protein Synthesis Inhibitors
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Receptors, Immunologic
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Receptors, KIR
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Receptors, NK Cell Lectin-Like
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Concanavalin A
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Brefeldin A