Abstract
The transcription factor NFAT (nuclear factor of activated T cells) controls the expression of many immunomodulatory proteins. African swine fever virus inhibits proinflammatory cytokine expression in infected macrophages, and a viral protein A238L was found to display the activity of the immunosuppressive drug cyclosporin A by inhibiting NFAT-regulated gene transcription in vivo. This it does by binding the catalytic subunit of calcineurin and inhibiting calcineurin phosphatase activity.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
African Swine Fever Virus / physiology*
-
Amino Acid Sequence
-
Animals
-
Binding Sites
-
Calcineurin / metabolism
-
Calcineurin Inhibitors*
-
Cell Nucleus / metabolism
-
Cells, Cultured
-
Chlorocebus aethiops
-
Cyclosporine / pharmacology
-
DNA-Binding Proteins / genetics
-
DNA-Binding Proteins / metabolism*
-
Genes, Reporter
-
Macrophages, Alveolar / virology*
-
Molecular Sequence Data
-
NF-kappa B / metabolism
-
NFATC Transcription Factors
-
Nuclear Proteins*
-
Recombinant Proteins / metabolism
-
Swine
-
Transcription Factors / genetics
-
Transcription Factors / metabolism*
-
Transcription, Genetic*
-
Vero Cells
-
Viral Proteins / genetics
-
Viral Proteins / metabolism*
Substances
-
A238L protein, African swine fever virus
-
Calcineurin Inhibitors
-
DNA-Binding Proteins
-
NF-kappa B
-
NFATC Transcription Factors
-
Nuclear Proteins
-
Recombinant Proteins
-
Transcription Factors
-
Viral Proteins
-
Cyclosporine
-
Calcineurin