[Western blot study of sera from patients with epidemic neuropathy]

Rev Cubana Med Trop. 1997;49(2):94-9.
[Article in Spanish]

Abstract

The immune response of a group of patients with epidemical neuropathy and of controls was studied by the immunoblotting technique against proteins of the Coxsackie virus and the proteins of slow effect isolated in our laboratory. 13 sera of patients with epidemical neuropathy and 9 sera of controls were studied. Of the 13 sera studied, 8 (61.5%) recognized protein VPI and 2 sera (15.3%) protein VP0 of the strain 47.93. Of the 9 controls studied, 4 (44.4%) recognized protein VPI and 3 (33.3%) protein VP0 only. With the antigen prepared from the slow effect strain it was obtained a specific signal in 5 (38.5%) sera of patients and in 2 sera (22.5%) of controls. It should be stressed that in this last case the protein observed had a molecular weight of 41,300 D, and that its size was smaller than that of the preceding protein detected against the strain 47.93 was of 45,000 D.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Antibodies, Viral / blood*
  • Antibody Specificity
  • Blotting, Western
  • Chlorocebus aethiops
  • Coxsackievirus Infections / epidemiology
  • Coxsackievirus Infections / immunology*
  • Coxsackievirus Infections / virology
  • Cuba / epidemiology
  • Disease Outbreaks*
  • Enterovirus / classification
  • Enterovirus / immunology*
  • Enterovirus / isolation & purification
  • Humans
  • Molecular Weight
  • Optic Nerve Diseases / blood
  • Optic Nerve Diseases / epidemiology
  • Optic Nerve Diseases / immunology*
  • Optic Nerve Diseases / virology
  • Peripheral Nervous System Diseases / blood
  • Peripheral Nervous System Diseases / epidemiology
  • Peripheral Nervous System Diseases / immunology*
  • Peripheral Nervous System Diseases / virology
  • Rabbits
  • Seroepidemiologic Studies
  • Vero Cells
  • Viral Proteins / chemistry
  • Viral Proteins / immunology*

Substances

  • Antibodies, Viral
  • Viral Proteins