Formation of a spherical multicellular aggregate (spheroid) of animal cells in the pores of polyurethane foam as a cell culture substratum and its application to a hybrid artificial liver

J Biomater Sci Polym Ed. 1998;9(7):765-78. doi: 10.1163/156856298x00136.

Abstract

Monkey kidney cells (Vero), human embryonic kidney cells (293), human liver cells (PLC/PRF/5), and primary rat, dog, and porcine hepatocytes formed spherical multicellular aggregates (spheroids) in the pores of polyurethane foam which was used as a cell culture substratum. These spheroids of various cell types express high cell activity for a long period. A practical hybrid artificial live support system composed of a multi-capillary polyurethane foam packed-bed type cell culture module including primary hepatocyte spheroids was developed. The success of the system is indicated by an 80% recovery rate in hepatic failure rats which died in control experiments.

MeSH terms

  • Animals
  • Cell Culture Techniques / methods
  • Cell Line
  • Chlorocebus aethiops
  • Dogs
  • Extracorporeal Circulation / methods
  • Humans
  • Kidney / cytology*
  • Liver / cytology*
  • Liver Failure / therapy
  • Liver, Artificial*
  • Polyurethanes / chemistry*
  • Porosity
  • Rats
  • Spheroids, Cellular*
  • Swine
  • Vero Cells

Substances

  • Polyurethanes