Abstract
We examined the inhibitory effects of N-beta-alanyl-5-S-glutathionyl-3,4-dihydroxyphenylalanine (5-S-GAD), a novel antibacterial substance from the immunized adult Sarcophaga peregrina (flesh fly), on protein phosphorylation using immune complexes of protein tyrosine kinases (PTKs) with anti-PTKs monoclonal antibody. We found that 5-S-GAD directly inhibited not only tyrosine phosphorylation of PTK p60(v-src), but also tyrosine phosphorylation of PTK p210(BCR-ABL). The inhibitory potency of 5-S-GAD was comparable to that of radicicol and herbimycin A of PTK inhibitor.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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3T3 Cells
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Animals
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Anti-Infective Agents / pharmacology*
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Dihydroxyphenylalanine / analogs & derivatives*
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Dihydroxyphenylalanine / pharmacology
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Diptera / chemistry
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Enzyme Inhibitors / pharmacology
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Fusion Proteins, bcr-abl / metabolism*
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Glutathione / analogs & derivatives*
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Glutathione / pharmacology
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Humans
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Mice
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Oncogene Protein pp60(v-src) / metabolism*
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Phosphorylation
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Protein-Tyrosine Kinases / antagonists & inhibitors
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Tumor Cells, Cultured
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Tyrosine / metabolism
Substances
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Anti-Infective Agents
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Enzyme Inhibitors
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N-beta-alanyl-5-S-glutathionyl-3,4-dihydroxyphenylalanine
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Tyrosine
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Dihydroxyphenylalanine
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Protein-Tyrosine Kinases
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Fusion Proteins, bcr-abl
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Oncogene Protein pp60(v-src)
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Glutathione