Detection of the leghemoglobin gene on two chromosomes of Phaseolus vulgaris by in situ PCR linked-fluorescent in situ hybridization (FISH)

T Uchiumi; R Kuwashiro; J Miyamoto; M Abe; S Higashi

doi:10.1093/oxfordjournals.pcp.a029436

Detection of the leghemoglobin gene on two chromosomes of Phaseolus vulgaris by in situ PCR linked-fluorescent in situ hybridization (FISH)

Plant Cell Physiol. 1998 Jul;39(7):790-4. doi: 10.1093/oxfordjournals.pcp.a029436.

Authors

T Uchiumi¹, R Kuwashiro, J Miyamoto, M Abe, S Higashi

Affiliation

¹ Department of Chemistry and BioScience, Faculty of Science, Kagoshima University, Japan.

PMID: 9729902
DOI: 10.1093/oxfordjournals.pcp.a029436

Abstract

The leghemoglobin (Lb) gene on the metaphase chromosomes of Phaseolus vulgaris was amplified by in situ PCR. The amplified Lb gene could be detected on two chromosomes by fluorescent in situ hybridization (FISH) using the short Lb gene probe.

MeSH terms

Base Sequence
Chromosome Mapping*
DNA, Plant
Fabaceae / genetics*
In Situ Hybridization, Fluorescence
Leghemoglobin / genetics*
Molecular Sequence Data
Plants, Medicinal*
Polymerase Chain Reaction / methods*

Substances

DNA, Plant
Leghemoglobin

Associated data

GENBANK/AB004549