[Analysis of contact dermatitis by cytokines. III.--Sensitization and crossreaction of phenolic compounds]

Yakugaku Zasshi. 1998 Aug;118(8):324-31. doi: 10.1248/yakushi1947.118.8_324.
[Article in Japanese]

Abstract

We examined sensitization and crossreaction by the guinea pig maximization test (GPMT) with phenolic compounds. Skin specimens were collected from earlobes of BALB/c mice on 24 h after elicitation phase of contact hypersensitivity reaction (CHR) with phenolic compounds. The expression of cytokines of skin specimens was examined by the reverse transcriptase/polymerase chain reaction (RT-PCR) method. Consequently, phenolic compounds which showed positive reaction in GPMT expressed IL-2 and IFN-gamma on 24 h after elicitation, and some phenolic compounds showed marked crossreaction. Therefore, it was found that on several phenolic compounds, dimerization of these compounds from monomer to dimer decrease sensitization.

MeSH terms

  • Animals
  • Cross Reactions*
  • Cytokines / analysis
  • Cytokines / metabolism*
  • Dermatitis, Contact / immunology*
  • Dimerization
  • Eugenol / analogs & derivatives*
  • Eugenol / immunology*
  • Female
  • Guinea Pigs
  • Mice
  • Mice, Inbred BALB C
  • Reverse Transcriptase Polymerase Chain Reaction
  • Skin / immunology*
  • Skin Tests / methods

Substances

  • Cytokines
  • Eugenol
  • isoeugenol